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Image Search Results
Journal: iScience
Article Title: Early TRAIL-engagement elicits potent multimodal targeting of melanoma by CD34 + progenitor cell-derived NK cells
doi: 10.1016/j.isci.2023.107078
Figure Lengend Snippet:
Article Snippet: To characterize receptor expression profile on NK cells, the following antibodies were used: anti-CD45 KO (J.33), anti-CD56 APCA750 (N901), anti-NKG2D PE (ON72), anti-NKp44 PE (Z231), anti-NKG2A PE (Z199), anti- KIR2DL1 KIR2DS1 PE (EB6.B) (all from Beckman Coulter),
Techniques: Virus, Recombinant, Multiplexing, Activity Assay, Software
Journal: Cancers
Article Title: Chemotherapeutics Used for High-Risk Neuroblastoma Therapy Improve the Efficacy of Anti-GD2 Antibody Dinutuximab Beta in Preclinical Spheroid Models
doi: 10.3390/cancers15030904
Figure Lengend Snippet: Impact of chemotherapy on percentage of ( A ) cytotoxic NK cells of lymphocytes and NK-cell-specific activating receptors. ( B – E ) 5 × 10 6 PBMCs were treated for 24 h under cell culture conditions with either carboplatin (2 µg/mL, open circles), cisplatin (1 µg/mL, open triangles), etoposide (0.5 µg/mL, open squares), vincristine (0.05 µg/mL, open diamonds), or the cyclophosphamide metabolite (4-HPC, 1 µg/mL, open hexagons). After 72 h of culturing, cells were analyzed for NKp30, NKp44, NKp46, NKG2D, and CD226 expression, using flow cytometry. ( A ) Relative number of cytotoxic NK cells (CD3 − , CD56 dim ) in lymphocytes. ( B – E ) Geometric mean fluorescence intensity (gMFI) of respective activating receptor of cytotoxic NK cells after chemotherapy. Data represent at least four biological replicates. Means and SEM are indicated as black lines and error bars, respectively. For statistical analysis, repeated measures ANOVA with appropriate post hoc test was used; * p < 0.05 vs., ** p < 0.01 versus untreated control (medium).
Article Snippet: Incubation with the following antibodies in a total volume of 100 μL was conducted for 20 min at RT: CD3-VioGreen (REA613, 1:200), CD56-APC-Vio770 (REA196, 1:200),
Techniques: Cell Culture, Expressing, Flow Cytometry, Fluorescence, Control